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Thy1-gcamp6s mice

Webb4 apr. 2024 · We implanted the optical window into GP4.3 (Thy1-GCaMP6s) mice at E15.5 and observed neurotransmission among three cells, as shown in movie S5 (Fig. 2, ,I I and andJ J). The placenta is a barrier that impedes passage of chemicals such as drugs into the embryo, but the diffusion rates for molecules are difficult to assess ( 28 , 29 ). Webb17 nov. 2024 · Thy1-GCaMP6s (stock #024275, The Jackson Laboratory) were used for 2P calcium imaging of cortical neurons. We crossed these mice with CBA/CaJ mice (stock #000654, The Jackson Laboratory), which are known for exceptional hearing in these mice (Parham and Willott, 1988), to create Thy1-GCaMP6s X CBA mice.

Thy1-GCaMP6 transgenic mice for neuronal population imaging in …

WebbTo circumvent this problem, we crossed the Thy1-GCaMP6s line to the CBA/CaJ line, which retains excellent hearing into adulthood, and confirmed that high-frequency ABR thresholds in Thy1-GCaMP6s × CBA mice were lower than the Thy1-GCaMP6s at all ages (Wilcoxon rank-sum test, P < 0.0005 for all ages) and did not change through 20 weeks ... WebbThy1-GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), which increases mRNA stability and protein expression [24,25]. Genotyping primers were 59-CATCAGTGCAGCA- GAGCTTC-39 (forward, anneals to calmodulin sequence in mary\u0027s french quarter https://uptimesg.com

Generation of a hybrid AppNL-G-F/NL-G-F×Thy1-GCaMP6s+/

Webb24 sep. 2014 · We tested Thy1-GCaMP6 mice for in vivo imaging in anesthetized and awake, behaving mice. We observed robust signals … Webb18 okt. 2012 · The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, … Webb18 okt. 2012 · In Thy1-GCaMP2.2c mice, we observed GCaMP-expressing neurons but could not detect activated cells within an imaging window of 250 × 250 μm during a 10 … huw gulliver cardiff

Parallel Processing of Sound Dynamics across Mouse

Category:Parallel Processing of Sound Dynamics across Mouse

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Thy1-gcamp6s mice

Intravital imaging of mouse embryos - PMC - National Center for ...

Webb16 sep. 2024 · The thick trace shows the mean for n = 6 mice. h, Change in power of the 1–3-Hz rhythm after administration of PCP, MK801 and memantine. The mean power at 1–3 Hz is shown ( n = 5 mice per... Webb25 feb. 2024 · Transgenic Thy1-GCaMP6s mice were administered three doses of propofol during P7–P11 and assessed at 4 months of age. Immunostaining in the motor cortex revealed a 10–18% reduction in the density of NeuN + cells and GCaMP6 + pyramidal neurones in both L2/3 and L5 of the motor cortex after repeated propofol exposure ( Fig. …

Thy1-gcamp6s mice

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WebbCommon Name: GP4.3. These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE) Project, express the green fluorescent calcium indicator, … Webb15 mars 2024 · To generate GCaMP6 transgenic mice, we utilized the previously described GCaMP6 fast and slow cDNA constructs (referred as GCaMP6F and GCaMP6S, …

Webb21 jan. 2024 · The experiment was performed on transgenic female mice (3- to 4-months old) expressing the calcium indicator GCaMP6s driven by the Thy1 promoter (Thy1-GCaMP6s; Jackson mouse #024275, GP4.3, C57BL/6-Tg (Thy1-GCaMP6s)GP4.3Dkim/J16). Webb024276 C57BL/6J-Tg(Thy1-GCaMP6f)GP5.5Dkim/J These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE ) Project, express the green …

WebbThy1-GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … Webb24 sep. 2014 · Here we present the generation and characterization of transgenic mice expressing the GECIs GCaMP6s or GCaMP6f under the Thy1 promoter. We quantified GCaMP6 expression across brain regions...

Webb22 juni 2024 · These differences may be expected, as the Thy1-GCaMP6s GP4.3 mice express GCaMP6s in a subset of excitatory cells, 16 and mice expressing GCaMP6s under the CaMKIIa promoter (Ai94, tetO) have GCaMP expressed in excitatory neurons, 50 while the AAV-PHP.eB-hSyn-GCaMP6s mice express GCaMP in all neurons. 51 For this reason, …

Webb24 sep. 2014 · Thy1-GCaMP6 transgenic mice for neuronal population imaging in vivo. Dana H1, Chen TW1, Hu A1, Shields BC1, Guo C1, Looger LL1, Kim DS1, Svoboda K1 … huw gulliverWebb15 mars 2024 · Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of the Thy1.2 promoter. Results These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in multiple brain regions. huw hedges royal society of chemistryWebb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory … huw gwytherWebb18 dec. 2013 · For widefield imaging experiments, the Thy1-GCaMP6s mice were anesthetized with isoflurane (5% for induction; 1.5-2% for maintenance) and placed on a stereotaxic frame with a heating pad. A... huw halse architectWebbThy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … huwh9 mail.sysu.edu.cnWebbWe quantified GCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for … hu whang teahuw higginson photo