Webb4 apr. 2024 · We implanted the optical window into GP4.3 (Thy1-GCaMP6s) mice at E15.5 and observed neurotransmission among three cells, as shown in movie S5 (Fig. 2, ,I I and andJ J). The placenta is a barrier that impedes passage of chemicals such as drugs into the embryo, but the diffusion rates for molecules are difficult to assess ( 28 , 29 ). Webb17 nov. 2024 · Thy1-GCaMP6s (stock #024275, The Jackson Laboratory) were used for 2P calcium imaging of cortical neurons. We crossed these mice with CBA/CaJ mice (stock #000654, The Jackson Laboratory), which are known for exceptional hearing in these mice (Parham and Willott, 1988), to create Thy1-GCaMP6s X CBA mice.
Thy1-GCaMP6 transgenic mice for neuronal population imaging in …
WebbTo circumvent this problem, we crossed the Thy1-GCaMP6s line to the CBA/CaJ line, which retains excellent hearing into adulthood, and confirmed that high-frequency ABR thresholds in Thy1-GCaMP6s × CBA mice were lower than the Thy1-GCaMP6s at all ages (Wilcoxon rank-sum test, P < 0.0005 for all ages) and did not change through 20 weeks ... WebbThy1-GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), which increases mRNA stability and protein expression [24,25]. Genotyping primers were 59-CATCAGTGCAGCA- GAGCTTC-39 (forward, anneals to calmodulin sequence in mary\u0027s french quarter
Generation of a hybrid AppNL-G-F/NL-G-F×Thy1-GCaMP6s+/
Webb24 sep. 2014 · We tested Thy1-GCaMP6 mice for in vivo imaging in anesthetized and awake, behaving mice. We observed robust signals … Webb18 okt. 2012 · The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, … Webb18 okt. 2012 · In Thy1-GCaMP2.2c mice, we observed GCaMP-expressing neurons but could not detect activated cells within an imaging window of 250 × 250 μm during a 10 … huw gulliver cardiff