WebApr 11, 2024 · Cholesterol uptake and efflux assays were already carried out by different groups, but the described methodology differs in regard to several assay conditions, i.e. differentiation of Caco-2 cells on plates with/without inserts, incubation with cholesterol packaged in micelles or not, composition of the micelles, incubation time and use/no use … WebJun 1, 1997 · The cholesterol content in the supernatant was determined on the Cobas Mira with the cholesterol reagent and calibrator from Sigma. LDL-C was determined by subtracting the supernatant cholesterol from the total serum cholesterol. D-LDL immunoseparation assay. The assay was performed as previously described .
Cholesterol Quantitation Kit - Sigma-Aldrich
WebApr 14, 2024 · BrdU assay revealed that cellular proliferation was significantly decreased in two clones of T47D: ... 0.1 µM of AKT inhibitor VIII (CS-0001, Funakoshi), 1 mg/ml of Cholesterol-Water Soluble (C4951-30MG; Sigma-Aldrich), and 1–25 µM of T0901317 (71810, Cayman Chemical) 24 h after plating. Webguide for assessing which protein assay(s) might be most effective with a given sample type. Use this information as a guide only. It is the researcher’s responsibility to validate the compatibility of specific sample buffers. Even minor changes in buffers and components affect each assay method to some extent. titan white octane
Cholesterol Quantification Assay Kit for colorimetric or …
WebCholesterol. Sigma Grade, ≥99%. View Price and ... Properties. description. Sphingomyelin (Milk, Bovine) assay >99% (TLC) form. powder. packaging. pkg of 1 × 200 mg (860063P-200mg) pkg of 1 × 25 mg ... it is now appreciated that sphingomyelin has a high affinity for cholesterol and that these two lipids pack tightly into liquid-ordered ... WebCholesterol Uptake Assay Kit sufficient for 100 fluorometric tests; find Sigma-Aldrich-MAK325 MSDS, related peer-reviewed papers, technical documents, similar products & … Web3. NP40 Substitute Assay Reagent (5X) - (Item No. 700024) This vial contains 12 ml of a 5X surfactant solution. Prior to use, mix the contents of the vial with 48 ml HPLC grade water. This diluted NP40 Substitute Assay Reagent is used to prepare tissue samples and can be used to prepare the standard curve. The diluted NP40 Substitute Assay Reagent titan white