WebMay 15, 2024 · PFGE was performed in a 1% agarose gel with 0.52 XTBE buffer (Tris-borate-EDTA) at 8 °C in a Rotaphor (Biometra). The voltage was set at 200 to 150 V/cm, and the pulse times ranged from 10 to 100 s (log). The pulse angle ranged from 120 to … WebOct 19, 2006 · The new Rotaphor 6.0 is a powerful system for the separation of high molecular DNA. Molecules of se-veral Mega bases can be separated in an Agarose gel. In contrast to conventional PFGE systems with fixed electrodes, the Rotaphor 6.0 features a patented electrode Rotor that allows for truly free positioning of a homogeneous electrical …
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WebThe Rotaphor System 8.0 Pulsed Field Gel Electrophoresis System allows the separation of large DNA molecules, beyond the 50kb limit of conventional agarose electrophoresis. This … http://www.labset.rs/rotaphor-6-0.html permit lee county
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WebMay 7, 2024 · Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (BioRad Laboratories) in 0.5x Tris-borate/EDTA buffer in either a Rotaphor (Biometra, Analitik Jena) or a CHEF Mapper XA (1703672, BIO-RAD) apparatus using the following protocol: block I: 9 h, 120° included angle, 5.5 V/cm, 30 to 18-s switch; block II: 6 … WebInnovation · Advancement · Development ELECTROPHORESIS Pulsed Field Rotaphor System 6.0 Order Information Item Order No. Rotaphor 6.0 System, 230 V, PC with Windows® … WebSample Block Block capacity Proposed sample volume Maximum Heating Average Heating Maximum Cooling; Biometra TAdvanced 384 G, 230 V 846-2-070-214: Aluminum, special … permit letter of authorization