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Mcherry-gfp-lc3b

Web2 jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to … WebTo monitor the autophagic sequestration of p62- and LC3-positive bodies, we developed a novel pH-sensitive fluorescent tag consisting of a tandem fusion of the red, acid-insensitive mCherry and the acid-sensitive green fluorescent proteins. This approach revealed that p62- and LC3-positive bodies are degraded in autolysosomes.

Septins and K63 ubiquitin chains are present in separate bacterial ...

WebThe mCherry-EGFP-LC3B-expressing cells were seeded in 24-well flat-bottom plates at a density of 120,000 cells/well and on the next day treated as indicated for 24 h. The FACS experiments were performed on a FACS BD LSR II (BD Biosciences, San Jose, CA, USA) using BD FACSDiva software. Web27 aug. 2024 · The mCherry-EGFP-LC3B fluorescence microscopy assay. Cells were seeded in 24-well plates (5 × 10 4 cells/well) one day before transfection. linear scaling relationship https://uptimesg.com

Neutrophil extracellular traps-triggered impaired autophagic flux …

Web19 feb. 2024 · Flow cytometry software like Flow Jo can run a ratiometric calculation as a derived parameter, where autophagic flux is the mCherry signal divided by the GFP signal. This assay is simple and quick with a 1 … Web14 okt. 2024 · Ad-mCherry-GFP-LC3B was used to demonstrate blockage of the autophagic flux in eukaryocytes . Infection of Ad-mCherry-GFP-LC3B resulted in overexpression of tandem mRFP/mCherry-GFP proteins. In cells lacking an autophagic state, mCherry-GFP-LC3B was present in the cytoplasm as diffuse yellow fluorescence … WebBeyotime adenovirus expressing mcherry gfp lc3b fusion protein recognizing cd46 Adenovirus Expressing Mcherry Gfp Lc3b Fusion Protein Recognizing Cd46, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more linear scaling normalization

pBABE-puro mCherry-EGFP-LC3B - biofeng.com

Category:Addgene: pCDH-EF1a-mCherry-EGFP-LC3B

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Mcherry-gfp-lc3b

知识分享：mCherry-GFP-LC3B自噬双标腺相关病毒产品说明

WebKEYWORDS pBABE-puro mCherry-EGFP-LC3B SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6952) AUTHORS N'Diaye EN, Kajihara KK, Hsieh I, Morisaki H, Debnath J, Brown EJ TITLE PLIC proteins or ubiquilins regulate autophagy-dependent cell survival during nutrient starvation. WebpCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry和绿色荧光蛋白GFP双重标签的LC3B的融合蛋白，呈现明亮的

Mcherry-gfp-lc3b

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WebWhen mCherry and GFP co-label LC3B into the lysosome, GFP is quenched by the acidic environment of the lysosome, mCherry still emits red fluorescence. Co-localization efficiency of mCherry signals of GFP and LC3 puncta was measured by Image J software and shown as a percentage of yellow dots in total number of mCherry puncta. 2.17. WebLenti-mCherry-EGFP-LC3B (C3002)和Lenti-EGFP-LC3B (C3003)适合用于一些腺病毒较难感染或者需要筛选稳定细胞株的情况；Ad-mCherry-GFP-LC3B (C3011)和AdPlus-mCherry-GFP-LC3B (C3012)由于表达起始时间短，适合用于能被腺病毒感染并且希望快速获得实验结果的情况，也可以在表达E1的HEK293A、HEK293等适当细胞中扩增，便于后续 ...

Web27 dec. 2024 · mCherry-GFP-LC3B慢病毒. mCherry-GFP-LC3B腺相关病毒. 四．自噬双标系统的工作原理. 未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表 … WebAd-mCherry-GFP-LC3B 关键词：Ad-mCherry-GFP-LC3B,YT160,百奥莱博 ·Tubulin抗体 编号：YT156 英文名称：Tubulin antibody 规格：>40次 本Tubulin抗体为进口分装，用纯化的微管蛋白作为抗原制备而成的抗Tubulin小鼠单克隆抗体。克隆号为B-5-1-2。本Tubulin抗体识别α-tubulin的C-terminal。

WebGreen fluorescent protein (GFP)-LC3B adenovirus, GFP-red fluorescent protein (RFP)-LC3B adenovirus and mCherry-EGFP-LC3 adeno-associated virus were obtained from Hanbio (Shanghai, China). Cell Culture. The human HSC cell line LX-2 (Procell, Wuhan, China) was cultured in DMEM supplemented with 10% FBS at 37 °C and 5% CO 2. Web22 jul. 2024 · Amplify a fused eGFP-LC3B ORF from the pBabe-mCherry-eGFP-LC3B template and insert it into the pBob-eGFP vector, replacing the existing eGFP ORF: 1.1.1.

WebPlasmid pBABE-puro mCherry-EGFP-LC3B from Dr. Jayanta Debnath's lab contains the insert microtubule-associated protein 1 light chain 3 beta and is published in EMBO Rep. …

Web15 sep. 2024 · Main methods: The effects of M 4 IDP on proliferation, cell cycle and ROS production were determined by CCK-8 and flow cytometry assays. Annexin-V-FITC/PI, Hoechst 33258, MDC staining assays and Ad-mCherry-GFP-LC3B fluorescence assay were performed to investigate apoptosis and autophagy. linear scarring or atelectasis in the lingulahttp://www.bioon.com.cn/news/showarticle.asp?newsid=92825 hots battle creekWebAd-mCherry-GFP-LC3B，即adenovirus expressing mCherry-GFP-LC3B fusion protein，是一种可以表达mCherry-GFP-LC3B融合蛋白的腺病毒，可以用于感染细胞或组织后进行细胞自噬(autophagy)的检测。 LC3是酵母自噬关键蛋白ATG8在哺乳动物中的同源蛋 … linear scarring/atelectasisWebGFP-LC3单荧光和mCherry-GFP-LC3双荧光指示系统. 自噬形成时，GFP-LC3或mCherry-GFP-LC3融合蛋白转移至自噬体膜，在荧光显微镜下形成多个明亮的绿色或黄色荧光斑点 … linearscape architectsWeb3 apr. 2024 · Virus particles were collected after 48 h infection, filtered through a 0.22 μm membrane, added to HCT116 cells, and then incubated for 24 h at 37 °C. The medium was replaced and HCT116 cells were selected for puromycin tolerance (5 μg/mL) to obtain Caspase 3 knock-down, GFP-LC3B, or mcherry-EGFP-LC3B overexpression cells. linear scarring in left lower lobe of lungWebDescription. pCMV-mCherry-GFP-LC3B is a plasmid developed by Biyuntian to express the red mCherry and green GFP double-labeled human LC3B fusion protein in mammalian … hotsawpable keyboard plateWeb8 jul. 2024 · Inmortalized Human Chondrocytes (T/C28a2) were employed to develop an autophagy reporter cell line using LC3 as a fluorescent reporter (mCherry-EGFP-LC3B) for autophagy. Figure 1 shows the schematic workflow of the screening assay starting with the development of the autophagy reporter cell line in human chondrocytes (mCherry-EGFP … linear scar left lung base