WebCommercial silicone stretching chambers (4 cm 2, STB-CH-10, STREX) were passively coated with 50 µg/mL fibronectin (FC010, Merck Millipore, MA, USA) at 37 °C for 24 h. The human AF cells at passages 5–6 were seeded in the silicone chambers (1 × 10 5 cells per chamber in 5 mL growth medium) and cultured at 37 °C, 5% CO 2 for 3 days, unless ... WebJournal: Cell reports Article Title: Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence doi: 10.1016/j.celrep.2024.112024 Figure Lengend Snippet: KEY RESOURCES TABLE Article Snippet: Human Plasma Fibronectin, Merck Millipore, Cat# FC010. Techniques: …

Humanplasma Fibronectin-aufgereinigtes Protein - Sigma-Aldrich

WebFeb 4, 2024 · One day before the experiment, 5 µm-pore transwell inserts (3421, Corning, Kennebunk, ME, USA) were coated with 100 µg/ml fibronectin (FC010, Merck, Darmstadt, Germany) for 1 h at 37 °C, washed ... WebRecommended amount is 2-10 μg/cm2. 2.Reconstitute HFN at 1mg/mL and dilute to the desired concentration with physiological buffer. 3.Wet the surface of each culture vessel … new zealand video game ratings

ヒト血漿フィブロネクチン精製タンパク質 - Sigma-Aldrich

WebMerck-Millipore默克密理博为科学家在生命科学领域的研究提供更简便、高效而且经济的解决方案。拥有超过40000 余种产品，基本覆盖了生命科学研究、药物开发生产的各个环节，蛋白抽提，蛋白分析，蛋白纯化, 更多产品详情点击www.bnbiotech.com Merck-Millipore部分现 … WebApplications. This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro. 1.Determine the amount of HFN needed to … WebCitation: Chen, C.-N.; Chang, H.- I.; Yen, C.-K.; Liu, W.-L.; Huang, K.-Y. Mechanical Stretch Induced Osteogenesis on Human Annulus Fibrosus Cells through ... milky synovial fluid indicates quizlet