Easysep rbc depletion
WebThe third is more usable for smaller volumes as it is a bit more expensive, but will remove erythrocytes magnetically based on antibody-labeled magnetic particles. (for example EasySep RBC... WebFeb 3, 2015 · This video demonstrates how to isolate highly purified immune cells from up to 16 whole blood samples at once with EasySep™ Direct on the EasyEights EasySep™ Magnet. The protocol takes as...
Easysep rbc depletion
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WebFigure 1. EasySep™ RBC Depletion Reagent provides superior RBC clearance and similar cell yield compared to ammonium chloride lysis (NH 4 Cl). Whole blood samples from healthy donors were processed to remove RBCs using either the EasySep™ RBC Depletion Reagent (n=39, all magnet platforms) or NH 4 Cl (n=27). Left: After RBC … WebTo find the antibody that is right for you, start by selecting a research area or category below or go to the complete antibody product listing page to search by target antigen, host, reactive species, conjugate, application, cell type or area of interest. Alternatively, use our Antibody Finder Tool to quickly identify antibodies verified with ...
WebThe ability to separate RBCs from the other components of whole blood has a number of useful clinical and research applications ranging from removing RBCs from typical clinical blood draw, bone marrow transplants to transfusions of these RBCs to patients after significant blood loss. Web> 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation or lysis Up to 97% purity of isolated cells Fast, easy-to-use and column-free Isolated cells are untouched This kit targets non-T cells for removal with antibodies recognizing specific surface markers.
http://www.nwbiotec.com/index.php?g=Asset&m=Download&a=dw&smurl=246 WebJun 25, 2024 · Magnetic RBC depletion was performed using the EasySep RBC depletion kit (STEMCELL technologies), according to the manufacturer’s instructions. Briefly, 500 μl of whole blood was supplemented with 6 mM EDTA (final concentration) and 500 μl PBS + 2% FCS, and transferred into a 5 mL polystyrene round-bottom tube.
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lena thimonWebEasySep™ Buffer (Catalog #20144) may also be used. ... Typically, further RBC depletion is not required following cell isolation. If residual RBCs are visible in the isolated cell pellet following centrifugation after the end of the protocol, resuspend in a small volume (0.2 - 2.5 mL) of recommended medium or desired culture medium and place ... lena tholenWebEasySep™ Direct Human PBMC Isolation Kit ... 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis Fast, easy-to-use, and column-free ... † To minimize RBC contamination in the isolated cells, pour off the sample along a clean area of the tube (i.e. the opposite side to where the sample was poured ... lena tholinssonWebRBC-depleted nucleated cells are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture o,r DNA/RNA … lena thomsonWebMar 3, 2024 · Plasma from whole blood was isolated by centrifugation at 400 × g for 10 minutes at 4°C. To remove platelets, the isolated plasma was centrifuged at 4000 × g for 10 minutes at 4°C. Untouched circulating leukocytes were isolated using EasySep RBC Depletion Reagent (StemCell Technologies). lena thomanderWebMay 1, 2016 · RBCs were removed from four different types of samples (whole blood, leukopak, buffy coat and bone marrow) either magnetically using the EasySep™ RBC Depletion Reagent or by NH4Cl lysis. To... lena thunborgWebApr 7, 2024 · The pellet was resuspended in EasySep ™ Buffer (STEMCELL Technologies) and incubated with microbeads to deplete leukocytes and red blood cells using EasySep ™ Human CD45 Depletion Kit II and EasySep ™ RBC Depletion Reagent respectively (STEMCELL Technologies). lena thimmler