Biotin ctp
WebBiotin-14-dCTP is a dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, … WebOptimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11 …
Biotin ctp
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WebRoche ctp 11 biotin. Ctp 11 Biotin, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … WebJul 21, 2016 · 2-Biotin run-on 2× reaction mix. i. Transfer a 28-μl aliquot of NRO master mix to a microcentrifuge tube. ii. Add 5 μl each of biotin-11-CTP (1 mM) and biotin-11-UTP (1 mM) to the NRO master ...
http://www.delta-f.com/details/1159044 WebBiotin-14-CTP is a CTP analog that contains biotin attached at the N4-position via a 14-atom linker. It is provided as a 10 mM solution in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. The amount of material provided is sufficient for up to 20 transcription reactions (1 µg template) using T7 RNA
Web40035. Price. 553.79 USD. Size. 25 µL. Contact us. Ask 11-CTP 10 mM in pH7 5 TE 11-cytidine-5' triphosphate tetralithium salt selection audit. + Add to compare. WebFeb 23, 2004 · Biotin pull-down assays were carried out by incubating 40 μg of cytoplasmic fractions with 1 μg of biotinylated transcripts for 1 h at room temperature. Complexes were isolated with paramagnetic streptavidin-conjugated Dynabeads (Dynal, Oslo, Norway), and bound proteins in the pull-down material were analyzed by Western blotting by using ...
WebBiotin-11-dCTP (Biotin-11-2′-deoxycytidine-5′-triphosphate, tetralithium salt) can be enzymatically incorporated into DNA via nick translation, random priming, or 3′-end terminal labeling. The number 11 is the …
WebBiotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; … davi cabral bjjWebBio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA … bayaran redoneWebDescription: Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin … davi bíblia sagradaWebBiotin-11-CTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace CTP in the in vitrotranscription reaction for RNA labeling. Double-stranded DNA (i.e.,linearized … davi calazansWebBiotin-11-CTP, >95%. Life Sciences & Diagnostics A Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance … davi btaWebBiotin-14-CTP can be efficiently incorporated into RNA transcripts using T7, SP6, or T3 RNA polymerase and the appropriate template in the presence of ATP, GTP, UTP, and Biotin-14-CTP. The biotinylated RNA can be used as a probe in membrane-based or in situhybridizations. The amount of material provided is sufficient for up to 20 transcription ... bayaran ptptn melalui kwspWebNov 1, 2024 · In this study, we designed a biotinylated CTP affinity probe which harbors three components: CTP, a binding moiety, which specifically recognizes the active sites of CTP-binding proteins; biotin, an enrichment moiety, which facilitates the enrichment of target proteins; γ-aminobutyric acid, a linker, which connects the enrichment moiety to … davi cpad