Atkat1
WebAug 13, 2013 · Here we report the characterization of two inward rectifying shaker-like potassium channels, OsKAT2 and OsKAT3, expressed in guard cell of rice plants. While OsKAT2 showed typical potassium channel activity, like that of Arabidopsis KAT1, OsKAT3 did not despite high sequence similarity between the two channel proteins. Webwith water (Figure 2a). Considering AtKAT1 (a pure inward K+ channel in Arabidopsis) and AtAKT2 have been successfully and repeatedly characterized in Xenopus oocytes, we …
Atkat1
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WebAtKAT1 plays roles as a major channel to uptake K + in guard cell when stomata open in dicot model plant Arabidopsis. In a recent publication, we isolated 3 KAT-like potassium … WebFirst, we compared the amino acid sequences of OsKAT2 versus AtKAT1 and found that the 235th and 285th threonine of OsKAT2 corresponded to the 256th and 306th threonine of …
WebJan 1, 2013 · AtKAT1 plays roles as a major channel to uptake K+ in guard cell when stomata open in dicot model plant Arabidopsis. In a recent publication, we isolated 3 KAT … WebWe identified mutation target amino acids, T235 and T285 of OsKAT2, corresponding T256 and T306 of AtKAT1, through sequence comparisons between AtKAT1 and OsKAT2. …
WebAug 1, 2008 · Given that AtKAT1-mediated K + uptake did not show saturation in the substrate range measured, we were unable to estimate a K m for AtKAT1. To establish … WebMar 18, 2003 · A series of NubG-fusion proteins including membrane proteins of different compartments such as a the plasma membrane potassium channel AtKAT1 [ 25 ], the plasma membrane monosaccharide transporter AtSTP1 [ 26 ], a SEC12 homolog (AtStl2p) as an ER membrane protein [ 27 ], and orthologs of the soluble, but also partially …
WebFig. 3 Homo-oligomerization of LeAMT1;1 and also AtKAT1 in split ubiquitin assay. Interactions among different N ub G, N ub WT, and C ub fusions were detected via growth assay on a synthetic minimal medium lacking adenine and histidine but supplemented with 0.075 m m methionine. The empty C ub vector was used as a negative control.
WebMar 7, 2007 · In that study, transiently expressed AtKAT1 in tobacco was found in moderately detergent-resistant membrane fractions, possibly suggesting its location within lipid rafts. CONCLUSION. Future studies that delve deeper into the properties of the lipid raft-associated proteins should enable the discovery of what triggers the formation of … barbarian 5e dual wieldingWebPlanteome annotation page/> Annotation: KAT1 locus:2170468 AT5G46240 AT5G46240 KAT1 AtKAT1 potassium channel in Arabidopsis thaliana 1 MPL12.2 MPL12_2 barbarian 5e great axeWebUptake in AtKAT1-expressing yeast did not show saturation up to 5 mM K + , indicating a lower affinity for K + (Fig. 2C). After subtracting the K + uptake rate from vector controls, reciprocal... barbarian 5e featuresWebDec 11, 2013 · The Arabidopsis AtHKT1;1 and AtKAT1 were used as controls in the growth inhibition experiments (Hirsch et al. 1998). As shown in the left panel of Figure 7 A, the … barbarian 5e buildWebOct 8, 2013 · AtKAT1 plays roles as a major channel to uptake K (+) in guard cell when stomata open in dicot model plant Arabidopsis. In a recent publication, we isolated 3 KAT-like potassium channels in rice. We expressed them in CHO cell to identify electrophysiological characteristics of the channels. barbarian 5e featsWebSep 23, 2024 · AtKAT1 (AT1G04710), a peroxisome-located gene, fused with red fluorescent protein (RFP) was used as a colocalization marker protein ( Fig. 2B ). Cotransforming nEYFP-IQM1 and cEYFP empty vector or nEYFP empty vector and cEYFP-CAT2/3 were set as negative controls. barbarian 5e homebrew pathsWebGiven that AtKAT1-mediated K + uptake did not show saturation in the substrate range measured, we were unable to estimate a K m for AtKAT1. To establish that Rb + uptake … barbarian 5e guide wikidot